Sasson Y, Zeltser D, Rogowski O, Kassirer M, Maharshak N, Nissenkorn A, Leibovitz E, Halperin P, Sharvit D, Sorkine P, Arber N, Berliner S
Department of Internal Medicine D, Tel-Aviv Sourasky Medical Center, Sackler Faculty of Medicine, Tel-Aviv University, Israel.
J Med. 1998;29(3-4):217-29.
We adopted whole blood flow cytometry and direct labeling of the CD11b/CD18 and CD62L antigens to study the relationship between their expression and leukocytosis in patients with infection/inflammation, acute stress and healthy volunteers. Mean +/- S.D. channel fluorescence intensity of CD11b/CD18 antigen on peripheral blood polymorphonuclears did not differ between patients with infection/ inflammation (173+/-78) and controls (167+/-72), but was significantly (p = 0.04) reduced in stress (135+/-60). No correlation was found between CD11b/CD18 antigen level and either polymorphonuclears absolute number or serum C-reactive protein. A significant negative correlation was noted between CD62L antigen expression on polymorphonuclears and their absolute number. We assume that cells with increased CD11b/CD18 surface concentrations are retained in the capillaries and that part of the leukocytes in the peripheral blood are stressed leukocytes with reduced CD11b/CD18. Thus, leukocytes detected in peripheral blood are not necessarily the most "inflamed" ones.
我们采用全血流式细胞术以及对CD11b/CD18和CD62L抗原进行直接标记，以研究感染/炎症患者、急性应激患者及健康志愿者中这些抗原的表达与白细胞增多之间的关系。感染/炎症患者外周血多形核细胞上CD11b/CD18抗原的平均±标准差通道荧光强度（173±78）与对照组（167±72）相比无差异，但在应激患者中显著降低（135±60，p = 0.04）。未发现CD11b/CD18抗原水平与多形核细胞绝对计数或血清C反应蛋白之间存在相关性。多形核细胞上CD62L抗原的表达与其绝对计数之间存在显著负相关。我们推测，CD11b/CD18表面浓度增加的细胞滞留在毛细血管中，外周血中的部分白细胞是CD11b/CD18减少的应激白细胞。因此，在外周血中检测到的白细胞不一定是最“炎症化”的白细胞。
