Schuitmaker J J, de Koster B M, Elferink J G
Department of Ophthalmology, Leiden University Medical Center, The Netherlands.
Photochem Photobiol. 1998 Dec;68(6):841-5.
Bacteriochlorin a photodynamic therapy (BCA-PDT) caused inhibition of interleukin (IL)-8-activated neutrophil migration, at concentrations that did not induce membrane damage. Random migration and migration induced by other chemoattractants were also inhibited, indicating that the effect of BCA-PDT was not at the level of chemoattractant-receptor interaction but down stream. The BCA-PDT completely blocked superoxide production of phorbol ester-stimulated neutrophils indicating that superoxide production by neutrophils present in the tumor before and during BCA-PDT is not the cause of inactivation of tumor cells. Both type I and type II quenchers prevented inhibition by BCA-PDT but only in electroporated cells. Confocal laser scanning microscopy showed that the fluorescence of BCA was located inside the cell. These results show that the effects of BCA-PDT are intracellular and of a mixed type I/type II character and that the neutrophils present in the tumor during illumination probably do not contribute to tumor eradication by releasing reactive oxygen species.
细菌叶绿素a光动力疗法（BCA-PDT）在不引起膜损伤的浓度下可抑制白细胞介素（IL）-8激活的中性粒细胞迁移。随机迁移以及由其他趋化因子诱导的迁移也受到抑制，这表明BCA-PDT的作用并非在趋化因子-受体相互作用水平，而是在其下游。BCA-PDT完全阻断了佛波酯刺激的中性粒细胞的超氧化物产生，这表明在BCA-PDT之前和期间肿瘤中存在的中性粒细胞产生的超氧化物并非肿瘤细胞失活的原因。I型和II型猝灭剂均可阻止BCA-PDT的抑制作用，但仅在电穿孔细胞中有效。共聚焦激光扫描显微镜显示BCA的荧光位于细胞内。这些结果表明，BCA-PDT的作用是细胞内的，具有I型/II型混合特征，并且光照期间肿瘤中存在的中性粒细胞可能不会通过释放活性氧来促进肿瘤根除。
