Schuitmaker J J, Vrensen G F, van Delft J L, de Wolff-Rouendaal D, Dubbelman T M, de Wolf A
Department of Ophthalmology, Academic Hospital, Leiden, The Netherlands.
Invest Ophthalmol Vis Sci. 1991 Sep;32(10):2683-8.
To study the development of tissue and cell damage, the early morphologic changes induced by photodynamic therapy (PDT) with the new photosensitizer bacteriochlorin a (BCA) were investigated in Greene hamster melanoma implanted in the anterior eye chamber of white rabbits up to 24 hr after BCA-PDT, using light and electron microscopy. Immediately after BCA-PDT, intracellular spaces were enlarged, and blood vessels were clotted with swollen erythrocytes. By electron microscopy, it was found that some mitochondria had fused inner and outer membranes, and the cristae mitochondriales were affected. With time, the severity of the tissue and cell damage increased, leading to almost complete tumor necrosis after 24 hr. The direct mitochondrial damage and the vascular damage induced by BCA-PDT probably both contribute to tumor necrosis.
为研究组织和细胞损伤的发展过程，利用光镜和电镜，对白兔前房植入的格林仓鼠黑色素瘤进行了新型光敏剂细菌叶绿素a（BCA）光动力疗法（PDT）诱导的早期形态学变化的研究，观察时间长达BCA-PDT后24小时。BCA-PDT后立即出现细胞内间隙增大，血管内红细胞肿胀并形成血栓。通过电子显微镜发现，部分线粒体的内膜和外膜融合，线粒体嵴受到影响。随着时间推移，组织和细胞损伤的严重程度增加，24小时后导致肿瘤几乎完全坏死。BCA-PDT诱导的直接线粒体损伤和血管损伤可能都促成了肿瘤坏死。
