Wang X, Niu X, Cheng D
Department of Plastic Surgery, Third Affiliated Hospital of Beijing Medical University, P.R. China.
Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi. 1997 Sep;11(5):257-60.
For observation of the change of transforming growth factor-beta 1 (TGF-beta 1) gene expression in the process of skin wound healing, the following experiments were performed. Sixteen Wistar rats were chosen. At each side of the rat's back, a 1 cm x 1.5 cm middle-thick skin wound was made. After 3, 6, 9 and 12 days, the specimens were taken from the wounds. For each specimen, half of it was used for RNA extraction, and underwent dot blotting; and the other half was frozen immediately and underwent in situ hybridization. The probes were dig-labeled PDGF-BB cDNA probe and TGF-beta 1 probe. The results showed that TGF-beta 1 gene was expressed mainly in fibroblast, epithelial cell and capillary endothelial cell. The peak of TGF-beta 1 mRNA content was in the 6th day postoperatively. After that, the content of TGF-beta 1 decreased to normal. It was suggested that TGF-beta 1 gene expression was in close relation with healing process. TGF-beta 1 may play an important regulatory role in the skin wound healing.
为观察转化生长因子-β1（TGF-β1）基因在皮肤创伤愈合过程中的表达变化，进行了以下实验。选取16只Wistar大鼠，在大鼠背部两侧制作1cm×1.5cm的中厚皮肤创伤。在术后3天、6天、9天和12天，从创伤处取材。对于每个标本，一半用于RNA提取并进行斑点杂交；另一半立即冷冻并进行原位杂交。探针为地高辛标记的血小板衍生生长因子-BB（PDGF-BB）cDNA探针和TGF-β1探针。结果显示，TGF-β1基因主要在成纤维细胞、上皮细胞和毛细血管内皮细胞中表达。TGF-β1 mRNA含量的峰值出现在术后第6天。此后，TGF-β1的含量降至正常水平。提示TGF-β1基因表达与愈合过程密切相关。TGF-β1可能在皮肤创伤愈合中起重要的调节作用。
