Cao X, Shidao H, Yu J
Department of Orthopedic Surgery, General Hospital of Jinan Military Area, P. R. China.
Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi. 1997 Nov;11(6):321-4.
In order to observe the collateral sprouting capacity of the nerve trunk after end-to-side anastomosis, a window was made on the epineurim of the donor nerve through which the result of the end-to-end anastomosis of nerves could be compared. Sixteen SD rats were chosen and divided into 4 groups randomly. Group 1, the peroneal nerve was severed, the epineurium of the tibial nerve was fenestrated and sutured the peroneal stump with the tibial nerve by end-to-side neurorrhaphy. Group 2, operative procedures were almost the same as that of Group 1, but no fenestration on the epineurium was done. Group 3, the distal peroneal nerve stump was sutured in paralell with the tibial nerve without fenestration on the tibial nerve and Group 4, severed the peroneal nerve and sutured the stumps by end-to-end anastomosis immediately. The peroneal function index(PFI) and acetylcholine transferase (ChAT) activity were assessed and the histological examination was performed in all rats. The results showed: between group 1 and 2, there was no difference in PFI and ChAT activity (P > 0.05). The existence of collateral sprouting in all groups was proved by histological examination. Even in Group 3, there were plenty of nerve fibers turned into tiny myelined nerve fibers through collateral sprouting. But the ChAT activity in Group 1 was only two thirds of that in Group 4. It was suggested that the epineurial sheath did not influence the collateral sprouting of the nerve.
为观察端侧吻合后神经干的侧支芽生能力，在供体神经的神经外膜上制作一个窗口，通过该窗口可比较神经端端吻合的结果。选取16只SD大鼠，随机分为4组。第1组，切断腓总神经，在胫神经神经外膜开窗，将腓总神经残端与胫神经行端侧神经缝合。第2组，手术操作与第1组基本相同，但未在神经外膜开窗。第3组，将腓总神经远端残端与胫神经平行缝合，未在胫神经开窗，第4组，切断腓总神经并立即行端端吻合。评估所有大鼠的腓总神经功能指数（PFI）和乙酰胆碱转移酶（ChAT）活性，并进行组织学检查。结果显示：第1组和第2组之间，PFI和ChAT活性无差异（P＞0.05）。组织学检查证实所有组均存在侧支芽生。即使在第3组，也有大量神经纤维通过侧支芽生转变为细小的有髓神经纤维。但第1组的ChAT活性仅为第4组的三分之二。提示神经外膜不影响神经的侧支芽生。
