类固醇激素产生组织的线粒体制剂对胆固醇及胆固醇类似物的氧化作用。
Oxidation of cholesterol and cholesterol analogues by mitochondrial preparations of steroid-hormone-producing tissue.
作者信息
Arthur J R, Blair H A, Boyd G S, Mason J I, Suckling K E
出版信息
Biochem J. 1976 Jul 15;158(1):47-51. doi: 10.1042/bj1580047.
Abstract
The rate of oxidation of cholesterol and its analogues to pregnenolone (3beta-hydroxypregn-5-en-20-one) by various mitochondrial preparations was measured. Sterols with the cholest-5-en-3beta-ol ring system and saturated side chains of different lengths were converted into pregnenolone rat rates similar to that of cholesterol. This marked lack of mitochondrial specificity towards the steroid side chains is in direct contrast with the rat liver microsomal cholesterol 7alpha-hydroxylase, which has a high specificity for the side chain. Steroids that retain the ring system, but contain hydroxyl groups at various points in the side chain, are converted into pregnenolone at rates three to eight times higher than in cholesterol. The results are discussed with reference to current ideas on the mechanism of the side-chain cleavage of cholesterol. The results are discussed with reference to current ideas on the mechanism of the side-chain cleavage of cholesterol.
摘要
测定了各种线粒体制剂将胆固醇及其类似物氧化为孕烯醇酮（3β-羟基孕-5-烯-20-酮）的速率。具有胆甾-5-烯-3β-醇环系统和不同长度饱和侧链的甾醇以与胆固醇相似的大鼠速率转化为孕烯醇酮。线粒体对类固醇侧链明显缺乏特异性，这与大鼠肝脏微粒体胆固醇7α-羟化酶形成直接对比，后者对侧链具有高度特异性。保留环系统但在侧链不同位置含有羟基的类固醇转化为孕烯醇酮的速率比胆固醇高3至8倍。结合当前关于胆固醇侧链裂解机制的观点对结果进行了讨论。结合当前关于胆固醇侧链裂解机制的观点对结果进行了讨论。
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