Kim K H, Lee J G, Park J H, Shin Y G, Lee S K, Cho T H, Oh S T
College of Pharmacy, Kangwon National University, Chunchon, Korea.
Arch Pharm Res. 1998 Dec;21(6):651-6.
A sensitive high-performance liquid chromatographic (HPLC) method for the determination of aloesin in plasma was developed. After solid-phase extraction from plasma and derivatization of aloesin and compound AD-1, which was prepared from aloesin as a internal standard, with 9-anthroylnitrile in the presence of quinuclidine, the derivatives were separated on a inertsil ODS-3 column using acetonitrile/methanol/water (3:1:6) as a mobile phase, and detected fluorimetrically at 460 nm with excitation at 360 nm. The detection limit of aloesin was 3.2 ng/ml in plasma (S/N = 3).
建立了一种灵敏的高效液相色谱（HPLC）法测定血浆中的芦荟素。从血浆中进行固相萃取后，将芦荟素和由芦荟素制备的化合物AD-1作为内标，在奎宁环存在下与9-蒽腈进行衍生化反应，衍生物在inertsil ODS-3柱上以乙腈/甲醇/水（3:1:6）作为流动相进行分离，并在激发波长为360 nm时于460 nm处进行荧光检测。血浆中芦荟素的检测限为3.2 ng/ml（信噪比=3）。
