Yamagami S, Kawashima H, Endo H, Tsuru T, Shibui H, Kagawa Y, Hori J, Yamagami H, Isobe M
Department of Ophthalmology, Jichi Medical School, Tochigi, Japan.
Transplantation. 1998 Dec 15;66(11):1504-10. doi: 10.1097/00007890-199812150-00014.
Cytokine profile is a key in understanding the mechanisms of allograft rejection. Cytokine expression in the aqueous humor and the correlation between the aqueous humor cells and corneal infiltrating cells are not fully understood in corneal transplantation.
Orthotopic mouse corneal transplantation was performed using BALB/c (H2d) mice as recipients, and C3H/He (H2k) and BALB/c mice as donors for allografts and isografts, respectively. Immunocytochemistry was performed on aqueous humor cells. Corneal graft was studied immunohistochemically. Cytokine gene expressions of the cells infiltrating the aqueous humor and corneal grafts were determined by the semiquantitative reverse transcription and polymerase chain reaction method.
Interferon-gamma, interleukin (IL)-2, IL-4, and IL-10 were detected in the cells infiltrating the aqueous humor and corneal grafts at both the protein and gene expression levels. T helper 1 (Th1) cytokine expressions at the protein level, however, were consistently predominant in the rejected allografts compared to those of Th2 cytokines. The cytokine and surface marker profiles of the cells in the aqueous humor corresponded well to those of the cells infiltrating the corneal grafts. Cytokine protein and mRNA expression levels in the aqueous humor decreased rapidly.
Allorejection in corneal transplantation is Th1 cytokine-predominant. Infiltrating cells do not express Th2 cytokine so much in allograft rejection, as compared with Th1 cytokine. The cell infiltration patterns of the aqueous humor were well correlated with those of the cornea.
细胞因子谱是理解同种异体移植排斥机制的关键。在角膜移植中，房水中细胞因子的表达以及房水细胞与角膜浸润细胞之间的相关性尚未完全明确。
以BALB/c（H2d）小鼠作为受体，分别采用C3H/He（H2k）小鼠和BALB/c小鼠作为同种异体移植和同基因移植的供体，进行原位小鼠角膜移植。对房水细胞进行免疫细胞化学检测。对角膜移植片进行免疫组织化学研究。采用半定量逆转录聚合酶链反应法测定浸润房水和角膜移植片的细胞的细胞因子基因表达。
在浸润房水和角膜移植片的细胞中，在蛋白质和基因表达水平均检测到γ干扰素、白细胞介素（IL）-2、IL-4和IL-10。然而，与Th2细胞因子相比，在排斥的同种异体移植中，Th1细胞因子在蛋白质水平的表达始终占主导地位。房水中细胞的细胞因子和表面标志物谱与浸润角膜移植片的细胞的谱高度相符。房水中细胞因子蛋白和mRNA表达水平迅速下降。
角膜移植中的同种异体排斥以Th1细胞因子为主。在同种异体移植排斥中，浸润细胞与Th1细胞因子相比，Th2细胞因子表达较少。房水的细胞浸润模式与角膜的细胞浸润模式密切相关。
