Miyamoto S, Kowalska M A, Marcinkiewicz C, Marcinkiewicz M M, Mosser D, Edmunds L H, Niewiarowski S
Department of Surgery, University of Pennsylvania School of Medicine, Philadelphia, USA.
Thromb Haemost. 1998 Dec;80(6):982-8.
Platelet microparticles (PMP) were isolated from outdated platelets by a combination of differential centrifugation and gel filtration, and the concentration of PMP was expressed in the equivalent of GPIIb/IIIa complex measured by captured ELISA. PMP bound to isolated neutrophils and macrophages in a dose-dependent manner, but they did not bind to lymphocytes. Incubation of PMP with neutrophils did not activate these cells as measured by up-regulation of Mac-1, release of human granulocyte elastase, and calcium mobilization. Incubation of PMP with macrophages did not enhance IL-8 production and the oxygen burst but slightly and significantly increased production of MCP-1. After 10 min incubation of PMP with macrophages, an increase of GPIIb/IIIa antigen was observed suggesting that PMP may be endocytosed by macrophages. In conclusion, PMP bind to leukocytes, but, in contrast to activated platelets, do not play a significant role in leukocyte activation.
通过差速离心和凝胶过滤相结合的方法，从过期血小板中分离出血小板微粒（PMP），PMP的浓度以通过捕获ELISA法测定的相当于GPIIb/IIIa复合物的量来表示。PMP以剂量依赖性方式与分离出的中性粒细胞和巨噬细胞结合，但不与淋巴细胞结合。通过Mac-1上调、人粒细胞弹性蛋白酶释放和钙动员来衡量，PMP与中性粒细胞共孵育不会激活这些细胞。PMP与巨噬细胞共孵育不会增强IL-8的产生和氧爆发，但会轻微且显著地增加MCP-1的产生。PMP与巨噬细胞共孵育10分钟后，观察到GPIIb/IIIa抗原增加，提示PMP可能被巨噬细胞内吞。总之，PMP与白细胞结合，但与活化血小板不同，在白细胞激活中不发挥重要作用。
