Vanacker J M, Delmarre C, Guo X, Laudet V
Centre National de la Recherche Scientifique UMR 49, Ecole Normale Supérieure de Lyon, France.
Cell Growth Differ. 1998 Dec;9(12):1007-14.
Estrogen receptor related alpha (ERRalpha) is an orphan nuclear receptor that is closely related to the estrogen receptor. It is expressed in a variety of adult and embryonic tissues (in particular, at the onset of ossification), as well as in several osteoblastic cell lines. ERRalpha acts as a site-specific, cell-specific transcriptional activator. Here, we show that ERRalpha transactivates the promoter of the mouse osteopontin (OPN) gene, the product of which is a marker of the late stages of osteoblastic differentiation. This effect is cell specific and is exerted through derivatives of the ERRalpha response element. Overexpression of ERRalpha in three different osteoblast-like cell lines results in an elevation of the amount of OPN-corresponding mRNA. Therefore, OPN is a target gene for ERRalpha, pointing to the role of the latter in osteoblast differentiation.
雌激素受体相关α（ERRα）是一种与雌激素受体密切相关的孤儿核受体。它在多种成年和胚胎组织中表达（特别是在骨化开始时），以及在几种成骨细胞系中表达。ERRα作为一种位点特异性、细胞特异性的转录激活因子。在这里，我们表明ERRα可反式激活小鼠骨桥蛋白（OPN）基因的启动子，该基因的产物是成骨细胞分化后期的标志物。这种效应具有细胞特异性，并且是通过ERRα反应元件的衍生物发挥作用的。在三种不同的成骨细胞样细胞系中ERRα的过表达导致OPN对应mRNA量的增加。因此，OPN是ERRα的靶基因，这表明ERRα在成骨细胞分化中的作用。
