用于检测胃黏膜幽门螺杆菌的内镜尿素酶传感器系统。

Endoscopic urease sensor system for detecting Helicobacter pylori on gastric mucosa.

作者信息

Sato T, Fujino M A, Kojima Y, Ohtsuka H, Ohtaka M, Kubo K, Nakamura T, Morozumi A, Nakamura M, Hosaka H

机构信息

First Department of Medicine, Yamanashi Medical University, Yamanashi, Japan.

出版信息

Gastrointest Endosc. 1999 Jan;49(1):32-8. doi: 10.1016/s0016-5107(99)70442-6.

DOI:10.1016/s0016-5107(99)70442-6

PMID:9869720

Abstract

BACKGROUND

It is now accepted that the curing of Helicobacter pylori infection will result in healing of chronic active gastritis and will change the natural history of gastroduodenal ulcer disease. Both endoscopic observation and evaluation of H. pylori status of the stomach are necessary for diagnosis and treatment of such patients. We carried out a clinical evaluation of an endoscopic tube type urease sensor system for the detection of H. pylori on the gastric mucosa. The differential output of two pH-sensitive field effect transistors at the tip of the endoscopic tube reflects the pH change in a urea solution depending on the existence of urease.

METHODS

In vitro experiments and clinical evaluation of the system were performed. Fifty-one patients who were suspected to have a gastroduodenal disorder were examined for H. pylori infection with this system, using the combination of histologic and bacteriologic examinations and rapid urease test as the references.

RESULTS

Clinical sensitivity and specificity of this system were 26 of 28 (92.9%) and 22 of 23 (95.7%), respectively. A measurement at 1 site is completed in about 1 minute. Repetition of the procedure provides multi-site measurements.

CONCLUSIONS

The present system makes possible quick on-site detection of H. pylori under endoscopic observation, with satisfactory sensitivity and specificity.

摘要

背景

目前已公认，根除幽门螺杆菌感染可治愈慢性活动性胃炎，并改变胃十二指肠溃疡疾病的自然病程。对于此类患者的诊断和治疗，内镜观察以及对胃内幽门螺杆菌状态的评估均很有必要。我们对一种用于检测胃黏膜幽门螺杆菌的内镜管式尿素酶传感器系统进行了临床评估。内镜管尖端两个对pH敏感的场效应晶体管的差分输出，可根据尿素酶的存在反映尿素溶液中的pH变化。