Ratajczak J, Marlicz W, Rozmysłowicz T, Machalinski B, Ratajczak M Z
Department of Cell Pathology Pomeranian Medical Academy, Szczecin, Poland.
Ann Transplant. 1996;1(4):35-8.
We have compared the efficiency of the different strategies allowing for a long term storage of a human CD34+ bone marrow cells. Accordingly, the aliquots of CD34+ cells isolated from bone marrow were frozen using: controlled rate freezing equipment, or freezer unprogrammed in a -80 degrees C mechanical freezer. After freezing, CD34+ cells were subsequently stored for one month in a liquid nitrogen tank at -196 degrees C or in mechanical freezer at -80 degrees C. We have found that both the viability and the recovery of clonogeneic progenitors of CD34+ cells samples stored at different temperature were similar. Therefore, regarding the costs and simplicity, we recommend the unprogrammed freezing and storage of human CD34+ cells at -80 degrees C in a mechanical freezer as a convenient, inexpensive, and reliable method for storing marrow for transplantation. This data also indirectly indicate that the aliquots of the CD34+ cells can be shipped frozen on dry ice (-80 degrees C), and that these cells will maintain viability under this conditions. Furthermore, in this study we have confirmed validity of our earlier observation that human CFU-Meg progenitors are more sensitive to cryopreservation.
我们比较了不同策略用于长期储存人CD34+骨髓细胞的效率。相应地，从骨髓中分离出的CD34+细胞 aliquots 使用以下方法冷冻：程序控制速率冷冻设备，或在-80℃机械冷冻机中未编程的冷冻机。冷冻后，CD34+细胞随后在-196℃的液氮罐中或-80℃的机械冷冻机中储存一个月。我们发现，储存在不同温度下的CD34+细胞样本的克隆原性祖细胞的活力和回收率相似。因此，考虑到成本和简便性，我们建议在-80℃的机械冷冻机中对人CD34+细胞进行未编程冷冻和储存，作为一种方便、廉价且可靠的骨髓移植储存方法。该数据还间接表明，CD34+细胞 aliquots 可以在干冰（-80℃）上冷冻运输，并且这些细胞在这种条件下将保持活力。此外，在本研究中，我们证实了我们早期观察结果的有效性，即人CFU-Meg祖细胞对冷冻保存更敏感。
