Sensh T
Hokkaido Igaku Zasshi. 1976 May;51(3):251-63.
The relation between copper content and histologic changes in the brain under disturbed copper metabolism was studied. In this experiment, three groups of mongrel dogs were used, i.e. (1) Copper administration only, (2) Copper administration with Eck's fistula, (3) Normal control. The copper content in each brain tissue was measured by the atomic absorption spectrophotometry and routine histologic preparations with some special stains were examined. Following results were obtained. (1) In the group of longstanding copper administration, there were no remarkable histologic changes in the brain, and the significant increase of copper content was not demonstrated in the cortex, medulla and basal ganglia. (2) The group of copper administration with Eck's fistula was divided into two subgroups by the presence or absence of icterus. (a) Without Icterus: Diffuse gliosis and type II Alzheimer's neuroglia were observed and copper content in the brain increased up to 1.8 fold of that of normal control. (b) With Icterus: The histologic changes which were observed in the non-icteric group were more severe especially in the brain stem containing vacuolar degeneration, shrinkage and ghost appearance of the nerve cells, hemorrhages and demyelination, but there were no marked histologic changes in cortex and medulla. The copper content of the brian increased up to 2 fold or more of that of normal control. Copper was not demonstrated histochemically in all groups. The copper content in the cortex and basal ganglia was almost the same but that of medulla was half as much as that of the formers in every group of this experiment. The difference of the copper content between cortex which had no remarkable histologic changes, and basal ganglia which had aforementioned severe changes, was not proved, and also the difference between the groups with icterus and without icterus was not demonstrated. The copper content did not always correlate with the severity of the histologic changes. From these results, it can be suggested that copper does not play a significant role in pathogenesis of specific histologic changes of the brain.
研究了铜代谢紊乱时大脑中铜含量与组织学变化之间的关系。本实验使用了三组杂种狗，即：（1）仅给予铜；（2）给予铜并伴有埃克瘘；（3）正常对照。用原子吸收分光光度法测量各脑组织中的铜含量，并检查常规组织学标本及一些特殊染色。得到以下结果。（1）在长期给予铜的组中，大脑没有明显的组织学变化，皮质、髓质和基底神经节中铜含量也没有显著增加。（2）伴有埃克瘘给予铜的组根据有无黄疸分为两个亚组。（a）无黄疸：观察到弥漫性胶质细胞增生和Ⅱ型阿尔茨海默神经胶质细胞，大脑中的铜含量增加至正常对照的1.8倍。（b）有黄疸：在无黄疸组中观察到的组织学变化更严重，特别是在脑干，包括空泡变性、神经细胞萎缩和鬼影样改变、出血和脱髓鞘，但皮质和髓质没有明显的组织学变化。大脑中的铜含量增加至正常对照的2倍或更多。所有组中均未通过组织化学方法显示铜。在本实验的每组中，皮质和基底神经节中的铜含量几乎相同，但髓质中的铜含量是前两者的一半。没有明显组织学变化的皮质与有上述严重变化的基底神经节之间铜含量的差异未得到证实，有黄疸组和无黄疸组之间的差异也未得到证实。铜含量并不总是与组织学变化的严重程度相关。从这些结果可以推测，铜在大脑特定组织学变化的发病机制中不发挥重要作用。
