Creson T K, Monaco P J, Rasch E M, Hagardorn A H, Ferslew K E
Department of Anatomy and Cell Biology, James H. Quillen College of Medicine, East Tennessee State University, Johnson City 37614-0582, USA.
Electrophoresis. 1998 Nov;19(16-17):3018-21. doi: 10.1002/elps.1150191637.
Capillary ion analysis (CIA) is a form of capillary electrophoresis that uses the differential electrophoretic mobility of ions to perform a separation of an ionic mixture. Application of this technique for detection of lithium concentrations in plasma and tissues of Poecilia was the purpose of this investigation. CIA was performed using a 75 microm ID x 60 cm length fused-silica capillary and a run electrolyte of 67.7 mg hydroxyisobutyric acid (HIBA), 52.8 mg 18-crown-6-ether and 64 microL UV-CAT-1 reagent (4-methylbenzylamine) in a volume of 100 mL water (18 (M)omega) with a voltage of 20 kV using ultraviolet absorption detection at 214 nm. Migration times were: potassium, 2.98 min; calcium, 3.48 min; sodium, 3.60 min; barium (internal standard), 4.15 min and lithium, 4.26 min. Lithium and barium migration times were stable and reproducible. Correlation coefficients (r) between peak area ratios of lithium/barium for concentrations ranging from 0.1 to 2.0 mM were from 0.976 to 0.996. Coefficients of variation (CV) for lithium concentrations ranged from 4.07 to 15.71% between days and 4.38 to 7.76% within-day. Application of this methodology for determination of lithium concentrations in the plasma, brains and livers of fish dosed with lithium for 23 days are presented. CIA is applicable to analysis of lithium concentrations in biological fluids and tissues of fish.
毛细管离子分析（CIA）是一种毛细管电泳形式，它利用离子的不同电泳迁移率来分离离子混合物。本研究的目的是应用该技术检测食蚊鱼血浆和组织中的锂浓度。使用内径75微米、长度60厘米的熔融石英毛细管进行CIA，运行电解质为67.7毫克羟基异丁酸（HIBA）、52.8毫克18-冠-6-醚和64微升UV-CAT-1试剂（4-甲基苄胺），溶于100毫升水（18兆欧）中，在214纳米处使用紫外吸收检测，电压为20千伏。迁移时间分别为：钾，2.98分钟；钙，3.48分钟；钠，3.60分钟；钡（内标），4.15分钟；锂，4.26分钟。锂和钡的迁移时间稳定且可重复。锂浓度在0.1至2.0毫摩尔范围内时，锂/钡峰面积比的相关系数（r）为0.976至0.996。锂浓度的变异系数（CV）在不同日期之间为4.07至15.71%，日内为4.38至7.76%。本文介绍了该方法在测定经锂处理23天的鱼的血浆、脑和肝脏中锂浓度的应用。CIA适用于分析鱼类生物体液和组织中的锂浓度。
