Dodson R F, Huang J, Williams M G, Bruce J R, Hammar S P
Department of Cell Biology and Environmental Sciences, The University of Texas Health Center at Tyler, 75710, USA.
Arch Pathol Lab Med. 1998 Dec;122(12):1103-6.
To investigate possible asbestos contamination of paraffin and migration by asbestos fibers during the tissue-embedding process.
Three sample categories were included in the study: (1) commercially available paraffin samples; (2) procedural control samples, which were prepared by processing the paraffin through the use of standard solvents and instruments; and (3) samples taken from areas adjacent to embedded tissue and evaluated for migration of asbestos from the tissue into the surrounding paraffin. The analysis of collected material from all samples was performed with analytical transmission electron microscopy.
Only one extremely small tremolite fiber was found in any of the commercially available samples of paraffin. No asbestos fibers were found either in the procedural control samples or in the samples taken adjacent to the embedded lung tissue.
First, it was extremely unlikely that any of the commercial paraffin samples would have skewed data due to embedded tissue. Second, the processing and instrumentation was not found to contribute asbestos material to the paraffin during the preparations. Finally, embedded tissue that contained high numbers of fibers, both uncoated fibers and asbestos bodies, did not contribute asbestos to the adjacent paraffin.
研究石蜡中可能存在的石棉污染以及在组织包埋过程中石棉纤维的迁移情况。
该研究纳入了三类样本：（1）市售石蜡样本；（2）程序对照样本，通过使用标准溶剂和仪器对石蜡进行处理来制备；（3）取自包埋组织相邻区域的样本，评估石棉从组织向周围石蜡的迁移情况。对所有样本收集的材料进行分析透射电子显微镜检查。
在任何市售石蜡样本中仅发现一根极小的透闪石纤维。在程序对照样本或取自包埋肺组织相邻区域的样本中均未发现石棉纤维。
第一，极不可能有任何市售石蜡样本因包埋组织而使数据产生偏差。第二，在制备过程中未发现处理过程和仪器会向石蜡中添加石棉材料。最后，含有大量纤维（包括未包被纤维和石棉小体）的包埋组织不会向相邻石蜡中释放石棉。
