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[用过氧乙酸进行血清冷杀菌]

[Serum sterilization in the cold with peracetic acid].

作者信息

Sprössig M, Wutzler P, Schweizer H, Mücke H

出版信息

J Hyg Epidemiol Microbiol Immunol. 1976;21(2):157-63.

PMID:987107
Abstract

On the basis of broad antimicrobic effect including also bacterial spores, mycoplasmas and viruses, the peracetic acid suits to sterilize in cold. By means of the final 0,1 to 0.02% concentration of the peracetic acid acting for 30 min. up to 5 days, it succeeded in sterilizing the serum used to prepare culture medium for bacteria, mycoplasmas and tissue cultures. Growth controls shown at least as good results as were those in using filtered sera. Chemical sterilization by means of the peracetic acid leads to significant diminution of neutralization and complementfixation antibodies in serum.

摘要

基于包括细菌芽孢、支原体和病毒在内的广泛抗菌作用,过氧乙酸适合于低温灭菌。通过最终浓度为0.1%至0.02%的过氧乙酸作用30分钟至5天,成功地对用于制备细菌、支原体和组织培养基的血清进行了灭菌。生长对照显示出至少与使用过滤血清时一样好的结果。用过氧乙酸进行化学灭菌会导致血清中中和抗体和补体结合抗体显著减少。

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