Tavana A M, Drucker D B, Boote V
Microbiology Laboratory, Dental School, Manchester, UK.
J Appl Microbiol. 1998 Dec;85(6):1029-35. doi: 10.1111/j.1365-2672.1998.tb05268.x.
The aim of this study was to determine the extent to which phospholipid molecular species profiles are affected by different environmental factors in Porphyromonas asaccharolytica ATCC 25260T. Phospholipids were analysed by Fast Atom Bombardment Mass Spectrometry (FAB-MS) in negative-ion mode. Under standard growth conditions (37 degrees C, pH 7.0, 48 h), the most intense high mass anions were m/z 653 and 662. The latter is consistent with the expected presence of PE (30:0). The only changes in profiles were quantitative. These were compared using the Pearson Coefficient of Linear Correlation. The r-values for initial pH comparisons ranged from 0.82 (pH 7.0 vs pH 6.0) to 1.00 (pH 5.0 vs pH 8.0), for incubation period, from 0.86 (48 vs 72 h) to 0.97 (96 vs 168 h), and for temperature, from 0.57 (40 vs 37 degrees C) to 0.96 (37 vs 36 degrees C). Differences were also seen when plates were incubated in anaerobe jars as opposed to an anaerobic work station (r = 0.75). It is concluded that it is essential to standardize growth parameters, and to use an anaerobe jar or an anaerobe work station, but not both.
本研究的目的是确定不同环境因素对解糖卟啉单胞菌ATCC 25260T磷脂分子种类谱的影响程度。采用快原子轰击质谱法（FAB-MS）在负离子模式下分析磷脂。在标准生长条件下（37℃，pH 7.0，48小时），质量数最高的强阴离子为m/z 653和662。后者与预期存在的PE（30:0）一致。图谱中唯一的变化是定量的。使用皮尔逊线性相关系数对这些变化进行比较。初始pH比较的r值范围为0.82（pH 7.0对pH 6.0）至1.00（pH 5.0对pH 8.0），培养时间的r值范围为0.86（48对72小时）至0.97（96对168小时），温度的r值范围为0.57（40对37℃）至0.96（37对36℃）。与在厌氧工作站中培养平板相比，在厌氧罐中培养时也观察到差异（r = 0.75）。结论是必须标准化生长参数，并使用厌氧罐或厌氧工作站，但不能同时使用两者。
