Alberati-Giani D, Cesura A M
Pharma Division, F. Hoffmann La Roche Ltd, Basel, Switzerland.
Amino Acids. 1998;14(1-3):251-5. doi: 10.1007/BF01345271.
The regulation of the expression of indoleamine 2,3-dioxygenase (IDO) was studied in cloned murine macrophages (MT2) and microglial (N11) cells. Both cell lines express IDO and inducible nitric oxide synthase activity after interferon-gamma (IFN-gamma) stimulation. The regulation of IDO expression appears to differ in the two cell lines. Nitric oxide (NO) production negatively modulates the expression of IDO activity in IFN-gamma-primed macrophages, thereby indicating a cross-talk between the kynurenine and nitridergic pathways in these cells. Conversely, this down-regulation of IDO activity by NO does not occour in microglial cells. A differential regulation of IDO expression in the two cell lines was also observed with LPS and picolinic acid. Together with previous findings, these results indicate the existence of marked differences in the regulation of the expression of the kynurenine pathway enzymes between macrophages and microglial cells.
在克隆的小鼠巨噬细胞（MT2）和小胶质细胞（N11）中研究了吲哚胺2,3-双加氧酶（IDO）表达的调控。两种细胞系在γ干扰素（IFN-γ）刺激后均表达IDO并具有诱导型一氧化氮合酶活性。IDO表达的调控在这两种细胞系中似乎有所不同。一氧化氮（NO）的产生对IFN-γ预处理的巨噬细胞中IDO活性的表达具有负调控作用，从而表明这些细胞中犬尿氨酸和氮能途径之间存在相互作用。相反，在小胶质细胞中，NO对IDO活性的这种下调作用并不发生。用脂多糖（LPS）和吡啶甲酸也观察到了两种细胞系中IDO表达的差异调控。与先前的研究结果一起，这些结果表明巨噬细胞和小胶质细胞之间在犬尿氨酸途径酶表达的调控上存在明显差异。
