Martin S F, Hergenrother P J
Department of Chemistry and Biochemistry, University of Texas at Austin 78712, USA.
Bioorg Med Chem Lett. 1998 Mar 17;8(6):593-6. doi: 10.1016/s0960-894x(98)00071-7.
The novel phospholipid 2, which bears a tert-butyl moiety in place of the natural trimethyl ammonium group of phosphatidylcholine, has been enzymatically synthesized via a transphosphatidylation reaction mediated by phospholipase D. The change from the choline headgroup in 1 to the tert-butyl group in 2 reduced the efficiency of hydrolysis by the phosphatidylcholine-preferring phospholipase C from Bacillus cereus by a factor of greater than 10(3).
新型磷脂2通过磷脂酶D介导的转磷脂酰基反应酶促合成，其在磷脂酰胆碱的天然三甲基铵基团位置带有叔丁基部分。从1中的胆碱头部基团变为2中的叔丁基，使蜡状芽孢杆菌中偏好磷脂酰胆碱的磷脂酶C的水解效率降低了10³倍以上。
