2'-脱氧-2'(S)-乙炔基寡核苷酸：一种能选择性稳定寡腺苷酸与DNA互补链配对的修饰。

2'-Deoxy-2'(S)-ethinyl oligonucleotides: a modification which selectively stabilizes oligoadenylate pairing to DNA complements.

作者信息

Buff R, Hunziker J

机构信息

Department of Chemistry and Biochemistry, University of Bern, Switzerland.

出版信息

Bioorg Med Chem Lett. 1998 Mar 3;8(5):521-4. doi: 10.1016/s0960-894x(98)00060-2.

DOI:10.1016/s0960-894x(98)00060-2

PMID:9871610

Abstract

Oligonucleotides consisting of 2'-deoxy-2'(S)-ethinyl-thymidine,-uridine, and -adenosine have been prepared. Whereas the modified pyrimidine oligonucleotides uniformly lead to weaker pairing affinity with DNA and RNA complements, the corresponding adenine oligonucleotides show enhanced thermal stability in duplexes with complementary DNA and decreased stability with RNA.

摘要

已制备了由2'-脱氧-2'(S)-乙炔基胸腺嘧啶、尿嘧啶和腺嘌呤组成的寡核苷酸。虽然修饰的嘧啶寡核苷酸与DNA和RNA互补物的配对亲和力普遍较弱，但相应的腺嘌呤寡核苷酸与互补DNA形成的双链体热稳定性增强，而与RNA形成的双链体稳定性降低。