Guzaev A, Boyode B, Balow G, Tivel K L, Manoharan M
Department of Medicinal Chemistry, Isis Pharmaceuticals, Carlsbad, CA 92008.
Bioorg Med Chem Lett. 1998 May 5;8(9):1123-6. doi: 10.1016/s0960-894x(98)00028-6.
A novel solid-phase synthesis of 5'-radiolabeled oligonucleotides is described. The labeling reaction is carried out by the phosphoramidite method with the aid of [4,6-di-14C]-5'-dimethoxytritylthymidine building block 1. The feasibility of the method is demonstrated by preparation of 3'-phosphorylated dodecathymidylate phosphorothioate containing radiolabeled nucleoside at the 5'-terminus.
描述了一种新型的5'-放射性标记寡核苷酸的固相合成方法。标记反应借助[4,6-二-14C]-5'-二甲氧基三苯甲基胸苷结构单元1通过亚磷酰胺法进行。通过制备在5'-末端含有放射性标记核苷的3'-磷酸化十二胸苷酸硫代磷酸酯证明了该方法的可行性。
