Gemmell E, Grieco D A, Cullinan M P, Westerman B, Seymour G J
Immunopathology Laboratory, School of Dentistry, University of Queensland, Brisbane, Australia.
Oral Microbiol Immunol. 1998 Dec;13(6):355-61. doi: 10.1111/j.1399-302x.1998.tb00691.x.
FACS analysis was used to determine the expression of 15 T-cell receptor V beta families on CD4 and CD8 cells in Porphyromonas gingivalis specific T-cell lines established from eight P. gingivalis-positive adult periodontitis and seven P. gingivalis-positive healthy or gingivitis subjects. All 15 T-cell receptor V beta families were expressed by the T-cell lines, although a significantly higher proportion of the CD4 cells expressed the 5.2-3 V beta region compared with the other 14 families, including the 5.3 region, suggesting that it is the 5.2 family which is overexpressed. This was also true for the CD8 cells, with the exception of the 3.1 region in adult periodontitis T-cell lines and the 3.1, 13.1/13.3 and 21.3 regions in healthy or gingivitis lines. Between the two clinical groups, a significantly lower percentage of 13.1/13.3-positive CD8 cells was noted in the adult periodontitis lines compared with the healthy or gingivitis lines. There was a significant reduction in DNA synthesis by the lines in the presence of P. gingivalis outer membrane antigens and fixed irradiated lymphoblastoid cell lines compared with cultures containing untreated irradiated lymphoblastoid cell lines and in cultures containing anti-class II major histocompatibility complex antibody in comparison with all other cultures. The results of this study have shown that P. gingivalis preferentially induces the T-cell receptor V beta 5.2 family on CD4 and CD8 cells in P. gingivalis-specific T-cell lines and that activation of T cells by P. gingivalis outer membrane antigens may be by antigen-specific rather than superantigen activity.
流式细胞术分析用于确定从8例牙龈卟啉单胞菌阳性的成人牙周炎患者和7例牙龈卟啉单胞菌阳性的健康或牙龈炎患者建立的牙龈卟啉单胞菌特异性T细胞系中，CD4和CD8细胞上15个T细胞受体Vβ家族的表达情况。所有15个T细胞受体Vβ家族均在T细胞系中表达，尽管与包括5.3区域在内的其他14个家族相比，CD4细胞中表达5.2 - 3 Vβ区域的比例显著更高，这表明过表达的是5.2家族。CD8细胞也是如此，但成人牙周炎T细胞系中的3.1区域以及健康或牙龈炎T细胞系中的3.1、13.1/13.3和21.3区域除外。在两个临床组之间，与健康或牙龈炎T细胞系相比，成人牙周炎T细胞系中13.1/13.3阳性CD8细胞的百分比显著更低。与含有未处理的辐照淋巴母细胞系的培养物相比，以及与含有抗II类主要组织相容性复合体抗体的培养物相比，在含有牙龈卟啉单胞菌外膜抗原和固定辐照的淋巴母细胞系的情况下，这些细胞系的DNA合成显著减少。本研究结果表明，牙龈卟啉单胞菌在牙龈卟啉单胞菌特异性T细胞系的CD4和CD8细胞上优先诱导T细胞受体Vβ5.2家族，并且牙龈卟啉单胞菌外膜抗原对T细胞的激活可能是通过抗原特异性而非超抗原活性。
