Siqueira J F, Magalhães F A, Lima K C, de Uzeda M
Institute of Microbiology, Federal University of Rio de Janeiro, Brazil.
Oral Microbiol Immunol. 1998 Dec;13(6):368-72. doi: 10.1111/j.1399-302x.1998.tb00693.x.
The pathogenicity of obligate and facultative anaerobic bacteria commonly found in endodontic infections was tested using a mouse model. The capacity of inducing abscesses was evaluated seven days after subcutaneous injection of the bacteria in pure culture and in combinations with either Prevotella intermedia or Prevotella nigrescens. Nine of the fifteen bacterial strains tested were pathogenic in pure culture. No statistically significant differences were detected between these strains in pure culture and in mixtures with either P. intermedia or P. nigrescens. Synergism between the bacterial strains was only apparent when associating Porphyromonas endodontalis with P. intermedia or P. nigrescens. Histopathological examination of tissue sections from induced abscesses revealed an acute inflammatory reaction, dominated by polymorphonuclear leukocytes. Sections from the control group using sterile medium showed no evidence of inflammatory reaction.
利用小鼠模型测试了牙髓感染中常见的专性厌氧菌和兼性厌氧菌的致病性。在皮下注射纯培养细菌以及与中间普氏菌或变黑普氏菌混合培养的细菌七天后，评估诱导脓肿的能力。所测试的15种细菌菌株中有9种在纯培养时具有致病性。在这些纯培养菌株与中间普氏菌或变黑普氏菌的混合物之间未检测到统计学上的显著差异。仅当牙髓卟啉单胞菌与中间普氏菌或变黑普氏菌联合时，菌株之间的协同作用才明显。对诱导脓肿的组织切片进行组织病理学检查显示出以多形核白细胞为主的急性炎症反应。使用无菌培养基的对照组切片未显示炎症反应迹象。
