Roh S, Clarke I J, Xu R, Goding J W, Loneragan K, Chen C
Prince Henry's Institute of Medical Research, Clayton, Vic., Australia.
Neuroendocrinology. 1998 Dec;68(6):361-4. doi: 10.1159/000054385.
We have studied the direct effect of leptin on the secretion of GH from the anterior pituitary gland of the sheep. Using primary cultures of ovine pituitary cells, leptin (10(-9)-10(-7) M) treatment for 30 min did not affect basal or GHRH (10(-7) M)-stimulated GH secretion. Following treatment for 24 h, a dose of 10(-7) M leptin stimulated basal GH secretion. In contrast, doses of 10(-7) and 10(-8) M leptin inhibited GHRH-stimulated GH secretion after 24 h of treatment. These results suggest that leptin can have long-term effects on the somatotropes, but no acute effect. Furthermore, leptin appears to have opposite effects on basal and GHRH-stimulated GH secretion.
我们研究了瘦素对绵羊垂体前叶生长激素（GH）分泌的直接影响。使用绵羊垂体细胞原代培养物，用瘦素（10^(-9)-10^(-7) M）处理30分钟，并不影响基础或生长激素释放激素（GHRH，10^(-7) M）刺激的GH分泌。处理24小时后，10^(-7) M的瘦素剂量刺激基础GH分泌。相反，处理24小时后，10^(-7)和10^(-8) M的瘦素剂量抑制GHRH刺激的GH分泌。这些结果表明，瘦素对生长激素细胞可能有长期影响，但无急性影响。此外，瘦素对基础和GHRH刺激的GH分泌似乎有相反的作用。
