Plettner E, Khumtaveeporn K, Shang X, Jones J B
Department of Chemistry, University of Toronto, Ontario, Canada.
Bioorg Med Chem Lett. 1998 Sep 8;8(17):2291-6. doi: 10.1016/s0960-894x(98)00415-6.
The reaction between methanethiosulfonate reagents and cysteine mutants of subtilisin is quantitative and can be used to prepare chemically modified mutant enzymes (CMMs) with novel properties. The virtually unrestricted structural variations possible for CMMs presents a preparative and screening challenge. To address this, a rapid combinatorial method for preparing and screening the activities of CMMs has been developed.
甲硫基磺酸盐试剂与枯草杆菌蛋白酶的半胱氨酸突变体之间的反应是定量的，可用于制备具有新特性的化学修饰突变酶（CMMs）。CMMs可能存在的几乎不受限制的结构变化带来了制备和筛选方面的挑战。为解决这一问题，已开发出一种用于制备和筛选CMMs活性的快速组合方法。
