Leonida M D, Sobolov S B, Fry A J
Fairleigh Dickinson University, Chemistry Department, Teaneck, NJ 07666, USA.
Bioorg Med Chem Lett. 1998 Oct 20;8(20):2819-24. doi: 10.1016/s0960-894x(98)00519-8.
Electroenzymatic reduction of NAD+ to NADH for subsequent use in enzymatic synthesis has been carried out at carbon electrodes bearing lipoamide dehydrogenase (LiDH) immobilized under a Nafion film. The self-mediated electron transfer was made possible by an excess of flavin adenine dinucleotide (FAD) entrapped together with LiDH. Results were compared to those obtained with a similar electrode containing both LiDH and a polymeric form of FAD (pFAD) prepared by anodic polymerization of FAD.
已在负载有固定在Nafion膜下的硫辛酰胺脱氢酶（LiDH）的碳电极上进行了将NAD⁺电酶还原为NADH以供后续酶促合成使用的操作。与LiDH一起截留的过量黄素腺嘌呤二核苷酸（FAD）使自介导电子转移成为可能。将结果与通过FAD阳极聚合制备的含有LiDH和聚合物形式FAD（pFAD）的类似电极所获得的结果进行了比较。
