大环内酯生物合成：在委内瑞拉链霉菌中，单一的细胞色素P450，即PicK，负责产生甲基霉素、新甲基霉素和苦霉素所需的羟基化反应。
Macrolide biosynthesis: a single cytochrome P450, PicK, is responsible for the hydroxylations that generate methymycin, neomethymycin, and picromycin in Streptomyces venezuelae.
作者信息
Graziani E I, Cane D E, Betlach M C, Kealey J T, McDaniel R
机构信息
Department of Chemistry, Brown University, Providence, RI 02912-9108, USA.
出版信息
Bioorg Med Chem Lett. 1998 Nov 17;8(22):3117-20. doi: 10.1016/s0960-894x(98)00553-8.
PMID:9873687
Abstract
The final step in the biosynthesis of methymycin, neomethymycin, and picromycin is an hydroxylation, shown to be carried out by the cytochrome P-450 monooxygenase, PicK. Direct comparison of the relative Kcat/K(m) values for the two substrates, YC-17 and narbomycin, showed a threefold rate preference of picK for narbomycin.
摘要
美活霉素、新美活霉素和苦霉素生物合成的最后一步是羟基化反应，已证明该反应由细胞色素P-450单加氧酶PicK催化。对两种底物YC-17和纳罗霉素的相对催化常数与米氏常数比值（Kcat/K(m)）进行直接比较，结果表明PicK对纳罗霉素的催化速率比对YC-17的催化速率高两倍。
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