Lee H C, Ko Y H, Baek S B, Kim D H
Department of Chemistry, Pohang University of Science and Technology, Korea.
Bioorg Med Chem Lett. 1998 Dec 1;8(23):3379-84. doi: 10.1016/s0960-894x(98)00624-6.
We have detected an anhydride intermediate in the CPA catalyzed proteolytic reaction of Gly-Tyr. It appears that since the zinc-bound water molecule which is believed to attack the scissile amide carbonyl carbon in the hydrolysis reaction is excluded by the N-terminal amino group of Gly-Tyr, the carboxylate of Glu-270 becomes to attack the amide bond to generate the anhydride intermediate.
我们在CPA催化的Gly-Tyr蛋白水解反应中检测到一种酸酐中间体。由于在水解反应中被认为会攻击可裂解酰胺羰基碳的锌结合水分子被Gly-Tyr的N端氨基排除，Glu-270的羧酸盐似乎会攻击酰胺键以生成酸酐中间体。
