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衰老和雌激素对鸡卵巢中含免疫球蛋白细胞定位的影响。

Effects of ageing and oestrogen on the localization of immunoglobulin-containing cells in the chicken ovary.

作者信息

Barua A, Yoshimura Y, Tamura T

机构信息

Graduate School for International Development and Cooperation, Hiroshima University, Japan.

出版信息

J Reprod Fertil. 1998 Sep;114(1):11-6. doi: 10.1530/jrf.0.1140011.

Abstract

Immunoglobulins in the chicken ovary are important for transfer of immunity to chicks through the egg and for protection of the ovary from infection. The aim of this study was to examine the effects of ageing and oestrogen on the population of Ig-containing cells in the chicken ovary. The ovarian tissue of immature, young laying and old laying hens and that of immature birds treated with diethylstilboestrol (DES), progesterone or sesame oil (vehicle) was processed for paraffin wax sections. The sections were stained for IgG, IgM and IgA by an indirect immunostaining method and the population of cells positive for each Ig was analysed under a light microscope. The number of cells positive for IgG, IgM and IgA was significantly greater in the ovarian stromal tissue of young laying hens than in immature or old laying hens (P < 0.01). The number of IgG- and IgM-positive cells in the thecal layer of primary follicles of young laying hens was significantly greater than that in immature and old laying hens (P < 0.01) and there were significantly more (P < 0.05) IgA-positive cells in young laying hens than in immature birds. The number of IgG-, IgM- and IgA-positive cells was significantly (P < 0.01) greater in both the stromal tissue and the thecal layer of DES-treated birds than in the vehicle-treated birds. Progesterone had no significant effect (P < 0.05) on the population of Ig-positive cells. These results indicate that the number of Ig-positive cells increases as chickens mature and decreases with ageing, and that oestrogen may be involved in this process.

摘要

鸡卵巢中的免疫球蛋白对于通过卵将免疫力传递给雏鸡以及保护卵巢免受感染非常重要。本研究的目的是研究衰老和雌激素对鸡卵巢中含免疫球蛋白细胞群体的影响。将未成熟、年轻产蛋和老龄产蛋母鸡的卵巢组织以及用己烯雌酚(DES)、孕酮或芝麻油(载体)处理的未成熟鸡的卵巢组织制成石蜡切片。通过间接免疫染色法对切片进行IgG、IgM和IgA染色,并在光学显微镜下分析每种免疫球蛋白阳性细胞的群体。年轻产蛋母鸡卵巢基质组织中IgG、IgM和IgA阳性细胞的数量显著多于未成熟或老龄产蛋母鸡(P<0.01)。年轻产蛋母鸡初级卵泡膜层中IgG和IgM阳性细胞的数量显著多于未成熟和老龄产蛋母鸡(P<0.01),且年轻产蛋母鸡中IgA阳性细胞显著多于未成熟鸡(P<0.05)。DES处理鸡的基质组织和膜层中IgG、IgM和IgA阳性细胞的数量均显著多于载体处理鸡(P<0.01)。孕酮对Ig阳性细胞群体无显著影响(P>0.05)。这些结果表明,Ig阳性细胞的数量随着鸡的成熟而增加,随着衰老而减少,并且雌激素可能参与了这一过程。

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