Geissbühler D M, Friess A E, Stoffel M H
Department of Veterinary Anatomy, University of Bern Veterinary School, Switzerland.
J Reprod Fertil. 1998 Sep;114(1):103-10. doi: 10.1530/jrf.0.1140103.
Unidentified tubulovesicular profiles have been reported in the apical cytoplasm of boar caput epididymal principal cells in addition to vesicles considered to be involved in endocytosis and secretion. The main aim of the present study was to clarify the character of these organelles and to differentiate them from the endocytic apparatus. Glucose-6-phosphatase (G6Pase) activity was determined as the reporter enzyme of the endoplasmic reticulum (ER) and phosphotungstic acid was used to visualize carbohydrate moieties in both the proximal and distal caput. Phosphotungstic acid revealed the glycocalyx of the endocytic apparatus, which was similar in both regions studied, and also stained specific granules of the proximal caput. Glucose-6-phosphatase showed the tubulovesicular profiles to be sparsely granulated ER that was poorly developed in the proximal caput and very abundant in the apical cytoplasm of the distal caput principal cells. The function of such large amounts of sparsely granulated ER with corresponding G6Pase activity in caput epididymal principal cells is unknown.
除了被认为参与胞吞作用和分泌的小泡外，在公猪附睾头主细胞的顶端细胞质中还报道了未鉴定的小管泡状结构。本研究的主要目的是阐明这些细胞器的特征，并将它们与内吞装置区分开来。测定了葡萄糖-6-磷酸酶（G6Pase）的活性作为内质网（ER）的报告酶，并使用磷钨酸来观察附睾头近端和远端的碳水化合物部分。磷钨酸显示了内吞装置的糖萼，在所研究的两个区域中相似，并且还对附睾头近端的特定颗粒进行了染色。葡萄糖-6-磷酸酶显示小管泡状结构是稀疏颗粒状的内质网，在附睾头近端发育不良，而在附睾头远端主细胞的顶端细胞质中非常丰富。附睾头主细胞中如此大量具有相应G6Pase活性的稀疏颗粒状内质网的功能尚不清楚。
