Adkins S, Kao C C
Department of Biology, Indiana University, Bloomington 47405, USA.
Virology. 1998 Dec 5;252(1):1-8. doi: 10.1006/viro.1998.9449.
Both the brome mosaic virus (BMV) and cowpea chlorotic mottle virus (CCMV) RNA-dependent RNA polymerases (RdRps) were found to recognize the BMV core subgenomic promoter in the same manner, requiring specific functional groups at positions -17, -14, -13, and -11 relative to the subgenomic initiation site (+1). For CCMV subgenomic RNA synthesis, both RdRps required the same nucleotides and four additional nucleotides at positions -20, -16, -15, and -10. The -20 nucleotide is partially responsible for the differential mode of recognition of the two promoters. These data provide evidence that the RNA can induce RdRps to alter the mode of promoter recognition.
人们发现，雀麦花叶病毒（BMV）和豇豆花叶病毒（CCMV）的RNA依赖性RNA聚合酶（RdRps）以相同方式识别BMV核心亚基因组启动子，相对于亚基因组起始位点（+1），在-17、-14、-13和-11位置需要特定的功能基团。对于CCMV亚基因组RNA合成，两种RdRps都需要相同的核苷酸以及在-20、-16、-15和-10位置的另外四个核苷酸。-20核苷酸部分负责两种启动子识别方式的差异。这些数据提供了证据，表明RNA可诱导RdRps改变启动子识别方式。
