Kim K H, Kim T K
College of Pharmacy, Kangwon National University, Chunchon, Korea.
Arch Pharm Res. 1998 Apr;21(2):217-22. doi: 10.1007/BF02974031.
A stereospecific HPLC method has been developed for the resolution of the enantiomers of salbutamol in human urine. After solid-phase extraction and derivatization with 2,3,4,6-tetra-O-acetyl-beta-D-glucopyranosyl isothiocyanate, the diastereomeric derivatives were resolved (Rs = 1. 83) on 5 microns octadecylsilan column using 35% acetonitrile in 0.05 M ammonium acetate buffer (pH = 6) as a mobile phase with electrochemical detection. The diastereomeric derivatives were formed within 30 min. The detection limit of each enantiomer was 20 ng/ml (S/N = 3).
已开发出一种立体专一性高效液相色谱法，用于分离人尿中沙丁胺醇的对映体。经固相萃取并用2,3,4,6-四-O-乙酰基-β-D-吡喃葡萄糖基异硫氰酸酯衍生化后，在5微米十八烷基硅烷柱上，以0.05M醋酸铵缓冲液（pH = 6）中35%的乙腈为流动相，采用电化学检测，分离了非对映体衍生物（Rs = 1.83）。非对映体衍生物在30分钟内形成。每个对映体的检测限为20 ng/ml（S/N = 3）。
