Kim K H, Lee J G, Kim D G, Kim M K, Park J H, Shin Y G, Lee S K, Jo T H, Oh S T
College of Pharmacy, Kangwon National University, Chunchon, Korea.
Arch Pharm Res. 1998 Oct;21(5):514-20. doi: 10.1007/BF02975367.
Simple and accurate methods to detect the adulteration of commercial aloe gel powder were developed. Crude polysaccharide in aloe gel powder was isolated by precipitating with excess ethyl alcohol and total hexose in isolated polysaccharide was determined by Dubois assay. After hydrolysis of non-dialysable polysaccharides, resultant free sugar was determined by gas chromatography for sugar recognition and ash contents was considered simultaneously. In some products, the content of ash was very low while the content of total hexose was very high. And polysaccharides of these products revealed typical dextran pattern, therefore, these products could be identified that adulterated with commercial maltodextrin. The content of maltodextrin in adulterated product was determined by HPLC and TLC analysis which could be adopted as a part of a certification process.
开发了简单准确的方法来检测市售芦荟凝胶粉的掺假情况。通过用过量乙醇沉淀分离芦荟凝胶粉中的粗多糖,并采用杜波依斯法测定分离出的多糖中的总己糖。在对不可透析多糖进行水解后,通过气相色谱法测定所得游离糖以识别糖类,并同时考虑灰分含量。在一些产品中,灰分含量非常低而总己糖含量非常高。并且这些产品的多糖呈现典型的右旋糖酐模式,因此,可以鉴定出这些产品掺有商业麦芽糊精。通过高效液相色谱法(HPLC)和薄层色谱法(TLC)分析测定掺假产品中麦芽糊精的含量,这可作为认证过程的一部分。