Wilson M J, Davis A, Ercole C, Pryor J L, Hensleigh H, Kaye K W, Dawkins H J, Wasserman N F, Reddy P, Ahmed K
Minneapolis Veteran's Administration Medical Center, University of Minnesota Cancer Center, USA.
J Androl. 1998 Nov-Dec;19(6):754-60.
Human prostatic secretion and seminal plasma contain certain protein kinase activities. Protein kinases play important roles in regulating a vast variety of cellular functions. The objective of this study was to determine whether one of these protein kinase activities in human prostatic secretion and seminal plasma is due to CK2, a messenger-independent, serine/threonine protein kinase that has considerable potential as a regulatory enzyme. By employing an anti-CK2 antibody and a CK2-specific peptide substrate, we have established that CK2 is present in these secretions. Approximately 70% of the CK2 activity present in seminal plasma of normozoospermic men (n = 49) is correlated to the number of sperm originally present in the semen. Further, both the prostate gland and the seminal vesicles are sources of CK2 activity in the seminal plasma of vasectomized men (n = 38). Although there was considerable variation between individuals in CK2 activity, the variation in repeat semen samples of the same vasectomized men (n = 6) was within 21%. There was no correlation of CK2 activity in seminal plasma with age for vasectomized (27-48 years, n = 38), oligozoospermic (28-43 years, n = 24), or normozoospermic men (26-48 years, n = 49). These data suggest that the majority of CK2 activity in the seminal plasma of normozoospermic men originates from sperm but that the prostate and seminal vesicles are accessory sex-gland sources of this enzyme.
人类前列腺分泌物和精浆含有某些蛋白激酶活性。蛋白激酶在调节多种细胞功能中发挥重要作用。本研究的目的是确定人类前列腺分泌物和精浆中的这些蛋白激酶活性之一是否归因于CK2，一种不依赖信使的丝氨酸/苏氨酸蛋白激酶，它作为一种调节酶具有相当大的潜力。通过使用抗CK2抗体和CK2特异性肽底物，我们已确定这些分泌物中存在CK2。正常精子男性（n = 49）精浆中约70%的CK2活性与精液中最初存在的精子数量相关。此外，前列腺和精囊都是输精管结扎男性（n = 38）精浆中CK2活性的来源。尽管个体之间的CK2活性存在相当大的差异，但同一输精管结扎男性（n = 6）的重复精液样本中的差异在21%以内。输精管结扎男性（27 - 48岁，n = 38）、少精子症男性（28 - 43岁，n = 24）或正常精子男性（26 - 48岁，n = 49）的精浆中CK2活性与年龄均无相关性。这些数据表明，正常精子男性精浆中的大部分CK2活性源自精子，但前列腺和精囊是该酶的附属性腺来源。
