Sasama J, Vyas B, Vukmanovic-Stejic M, Kemeny D M
Department of Immunology, Guy's, King's, and St Thomas's School of Medicine, London, UK.
Int Arch Allergy Immunol. 1998 Dec;117(4):255-62. doi: 10.1159/000024020.
The aim of this study was to compare the effects of IL-4, IL-12 and IFN-gamma on the production of T helper-1 (Th1) and T helper-2 (Th2)-type cytokines from human peripheral blood 'naive' CD45RA and 'memory' CD45RO CD4 T cells. CD45RA or CD45RO CD4 T cells were cultured for 4 days with phorbol myristate acetate (PMA) and ionomycin and either IL-4, IFN-gamma or IL-12 and their ability to proliferate and secrete IFN-gamma and IL-4 determined. Purified CD45RO CD4 T cells stimulated with PMA and ionomycin secreted higher levels of IL-4 and IFN-gamma, as measured by ELISA, than CD45RA CD4 T cells which secreted little IL-4 or IFN-gamma. However, CD45RA and CD45RO CD4 T cells proliferated to the same extent and IL-4, IFN-gamma and IL-12 had no effect on this. IL-12 and IFN-gamma had no effect on the amount of IL-4 secreted by PMA and ionomycin-stimulated CD45RO CD4 T cells, but culture with IL-4 enhanced IL-4 production 7-fold. IL-12 increased the amount of IFN-gamma produced by CD45RO CD4 T cells 2- to 3-fold. Small amounts of IFN-gamma production were induced in CD4 CD45RA T cells by IL-12 and IFN-gamma. These results indicate: (1) that CD45RA cells cannot make significant amounts of IL-4 under the conditions used, (2) that CD45RO cells can produce both Th1 and Th2 cytokines immediately upon restimulation, (3) that IL-12 favours Th1 cytokine production in both CD45RA and CD45RO CD4 T cells, and (4) that IFN-gamma favours IFN-gamma production in CD45RA but not CD45RO cells.
本研究的目的是比较白细胞介素-4（IL-4）、白细胞介素-12（IL-12）和干扰素-γ（IFN-γ）对人外周血“初始”CD45RA和“记忆”CD45RO CD4 T细胞产生辅助性T细胞1（Th1）型和辅助性T细胞2（Th2）型细胞因子的影响。将CD45RA或CD45RO CD4 T细胞与佛波酯肉豆蔻酸酯乙酸酯（PMA）和离子霉素以及IL-4、IFN-γ或IL-12一起培养4天，并测定它们的增殖能力以及分泌IFN-γ和IL-4的能力。通过酶联免疫吸附测定法（ELISA）测量，用PMA和离子霉素刺激的纯化CD45RO CD4 T细胞分泌的IL-4和IFN-γ水平高于分泌少量IL-4或IFN-γ的CD45RA CD4 T细胞。然而，CD45RA和CD45RO CD4 T细胞的增殖程度相同，且IL-4、IFN-γ和IL-12对此没有影响。IL-12和IFN-γ对PMA和离子霉素刺激的CD45RO CD4 T细胞分泌的IL-4量没有影响，但与IL-4一起培养可使IL-4的产生增加7倍。IL-12使CD45RO CD4 T细胞产生的IFN-γ量增加2至3倍。IL-12和IFN-γ在CD4 CD45RA T细胞中诱导产生少量的IFN-γ。这些结果表明：（1）在所用条件下，CD45RA细胞不能产生大量的IL-4；（2）CD45RO细胞在再次刺激后可立即产生Th1和Th2细胞因子；（3）IL-12有利于CD45RA和CD45RO CD4 T细胞中Th1细胞因子的产生；（4）IFN-γ有利于CD45RA细胞中IFN-γ的产生，但对CD45RO细胞没有影响。
