在中度嗜热菌嗜热葡萄糖苷芽孢杆菌12060菌株中，孕酮6-羟基化由细胞色素P-450催化。

Progesterone 6-hydroxylation is catalysed by cytochrome P-450 in the moderate thermophile Bacillus thermoglucosidasius strain 12060.

作者信息

Sideso O, Williams R A, Welch S G, Smith K E

机构信息

Department of Biochemistry, Queen Mary and Westfield College, London, UK.

出版信息

J Steroid Biochem Mol Biol. 1998 Oct;67(2):163-9. doi: 10.1016/s0960-0760(98)00101-0.

DOI:10.1016/s0960-0760(98)00101-0

PMID:9877217

Abstract

The moderate thermophile, Bacillus thermoglucosidasius, transforms progesterone into four metabolites. These are 6alpha- and 6beta-hydroxyprogesterone, androstenedione and testosterone. This is the first report of bacterial 6alpha-hydroxylation of steroids. The identity of the progesterone metabolites shows that there are three major types of transforming activity in this organism; C-17-C-20 lyase that cleaves the pregnane side chain of the substrate, C-17 oxidoreductase that interconverts the metabolites androstenedione and testosterone, and 6-hydroxylation. 6-hydroxylation activity was purified virtually to homogeneity and was shown to be catalysed by a cytochrome P-450 monooxygenase enzyme. This is the first report of a thermostable cytochrome P-450.

摘要

嗜温菌嗜热葡萄糖苷芽孢杆菌可将孕酮转化为四种代谢产物。它们分别是6α-和6β-羟基孕酮、雄烯二酮和睾酮。这是关于细菌对类固醇进行6α-羟基化的首次报道。孕酮代谢产物的特性表明，该生物体存在三种主要的转化活性；可裂解底物孕烷侧链的C-17-C-20裂解酶、可使代谢产物雄烯二酮和睾酮相互转化的C-17氧化还原酶以及6-羟基化作用。6-羟基化活性几乎被纯化至同质，并被证明是由一种细胞色素P-450单加氧酶催化的。这是关于热稳定细胞色素P-450的首次报道。