Nagy-Agren S E, Cooney E L
Infectious Diseases Section, VAMC, Salem, VA 24153, USA. nagy-agren.
J Infect Dis. 1999 Feb;179(2):493-6. doi: 10.1086/314596.
The effect of interleukin (IL)-12 on T lymphocyte function was assessed in 47 human immunodeficiency virus (HIV)-infected persons of different disease stages and 16 seronegative controls. Lymphoproliferative responses (LPR) were measured to various HIV and non-HIV antigens and mitogens using peripheral blood mononuclear cells cultured with or without IL-12. Without exogenous IL-12, 96% of HIV-seropositive persons responded to mitogens, 77% to >=1 non-HIV antigen, and 11% to >=1 HIV antigen. Supplementation with IL-12 augmented LPR of HIV-seropositive persons to non-HIV antigens; however, the effect was greatest for those with higher CD4 cells (40% vs. 9% for those with >200 vs. <=200 CD4 cells/mm3). Addition of IL-12 also enhanced LPR to HIV antigens in 30% of subjects. This effect was most pronounced for those with>500 CD4 cells/mm3 (56% [P<. 05]). These findings suggest that impaired T lymphocyte recognition of foreign antigen, including HIV, can be reconstituted in part for selected HIV-seropositive persons.
在47名处于不同疾病阶段的人类免疫缺陷病毒（HIV）感染者和16名血清阴性对照者中评估了白细胞介素（IL）-12对T淋巴细胞功能的影响。使用外周血单个核细胞，在有或无IL-12的情况下培养，测量对各种HIV和非HIV抗原及有丝分裂原的淋巴细胞增殖反应（LPR）。在没有外源性IL-12的情况下，96%的HIV血清阳性者对有丝分裂原产生反应，77%对≥1种非HIV抗原产生反应，11%对≥1种HIV抗原产生反应。补充IL-12可增强HIV血清阳性者对非HIV抗原的LPR；然而，对于CD4细胞较高的人这种作用最大（CD4细胞>200/mm3的人为40%，而≤200/mm3的人为9%）。添加IL-12还使30%的受试者对HIV抗原的LPR增强。这种作用在CD4细胞>500/mm3的人中最为明显（56%[P<0.05]）。这些发现表明，包括HIV在内的外来抗原的T淋巴细胞识别受损，对于部分选定的HIV血清阳性者可部分重建。
