García-Sastre A, Egorov A, Matassov D, Brandt S, Levy D E, Durbin J E, Palese P, Muster T
Department of Microbiology, Mount Sinai School of Medicine, One Gustave L. Levy Place, New York, New York, 10029, USA.
Virology. 1998 Dec 20;252(2):324-30. doi: 10.1006/viro.1998.9508.
The NS1 protein is the only nonstructural protein encoded by influenza A virus. It has been proposed that the NS1 performs several regulatory functions during the viral replication cycle, including the regulation of synthesis, transport, splicing, and translation of mRNAs. Through the use of reverse genetics, a viable transfectant influenza A virus (delNS1) which lacks the NS1 gene has been generated. Our results indicate that the NS1 of influenza A virus is an auxiliary (virulence) factor which plays a crucial role in inhibiting interferon-mediated antiviral responses of the host.
NS1蛋白是甲型流感病毒编码的唯一非结构蛋白。有人提出，NS1在病毒复制周期中发挥多种调节功能，包括对mRNA的合成、运输、剪接和翻译的调节。通过反向遗传学方法，已构建出一种缺乏NS1基因的可存活转染型甲型流感病毒（delNS1）。我们的结果表明，甲型流感病毒的NS1是一种辅助（毒力）因子，在抑制宿主的干扰素介导的抗病毒反应中起关键作用。
