Yoshimi Y, Yamazaki S, Ikekita M
Department of Applied Biological Science, Faculty of Science and Technology, Science University of Tokyo, 2641 Yamazaki, Noda, Chiba, Japan.
Biochim Biophys Acta. 1999 Jan 4;1426(1):69-79. doi: 10.1016/s0304-4165(98)00123-8.
The changes in Asn-linked oligosaccharide composition in the murine cerebrum during development have been examined by high-performance liquid chromatography (HPLC) and electrospray ionization mass spectrometry (ESI-MS). The oligosaccharides, obtained from murine cerebrum in several developmental stages, were separated by HPLC on anion-exchange and reverse-phase columns. We found that two Asn-linked oligosaccharides, designated oligosaccharide I and oligosaccharide II, had their expression changed during postnatal development. Whereas oligosaccharide I was reduced during brain development, oligosaccharide II was increased. The structures of oligosaccharides I and II were analyzed by ESI-MS and sequential exoglycosidase digestions. Judging from the molecular and fragment ions in each oligosaccharide, the oligosaccharide I was composed of 5Hex+2HexNAc+ABOE (MW 1467.2) and the oligosaccharide II was 3Hex+4HexNAc+DoHex+ABOE (MW 1695.2). The results of sequential exoglycosidase digestion indicated that the oligosaccharide I was an oligomannose type saccharide and the oligosaccharide II was a biantennary complex type saccharide including fucose. The proposed structures are shown below. These results offer an important clue to the role of Asn-linked oligosaccharides associated with development of the central nervous system.
利用高效液相色谱法（HPLC）和电喷雾电离质谱法（ESI-MS），研究了小鼠大脑在发育过程中与天冬酰胺连接的寡糖组成的变化。从处于几个发育阶段的小鼠大脑中获得的寡糖，通过HPLC在阴离子交换柱和反相柱上进行分离。我们发现，两种与天冬酰胺连接的寡糖，即寡糖I和寡糖II，在出生后的发育过程中表达发生了变化。在大脑发育过程中，寡糖I减少，而寡糖II增加。通过ESI-MS和顺序外切糖苷酶消化对寡糖I和II的结构进行了分析。从每种寡糖中的分子离子和碎片离子判断，寡糖I由5个己糖+2个N-乙酰己糖胺+ABOE组成（分子量1467.2），寡糖II由3个己糖+4个N-乙酰己糖胺+去氧己糖+ABOE组成（分子量1695.2）。顺序外切糖苷酶消化的结果表明，寡糖I是一种低聚甘露糖型糖类，寡糖II是一种含有岩藻糖的双触角复合型糖类。所提出的结构如下所示。这些结果为与中枢神经系统发育相关的天冬酰胺连接的寡糖的作用提供了重要线索。
