Rao H, Pio J, Kessler J P
Neurobiologie et Neurophysiologie Fonctionnelles, URA CNRS 1832, Case 351, Faculté Saint-Jérôme, F13397, Marseille, Cédex 20, France.
Brain Res Dev Brain Res. 1999 Feb 5;112(2):281-5. doi: 10.1016/s0165-3806(98)00178-3.
Synaptophysin (SY) is a major integral membrane protein of small synaptic vesicles. In the present study, SY immunohistochemistry was used to investigate the postnatal development of the rat nucleus tractus solitarii (NTS) and nucleus ambiguus/ventrolateral medulla (NA/VLM). Whatever the age of the animal, SY immunoreactivity showed a typical pattern of punctate staining reminiscent of presynaptic terminal labeling. In the NTS and the NA/VLM, SY immunoreactive puncta were few at birth and increased in number during the first postnatal days. These changes were quantified by measuring the volumetric fraction occupied by SY immunoreactive puncta at various postnatal ages. Using volumetric fraction data, an index of the total volume occupied SY immunoreactivity in each region was then calculated. Between birth and adulthood, this index increased by 6-fold in the NTS and by 7-fold in the NA/VLM, suggesting that most of the synaptic development of these regions occurs postnatally.
