Tang G Q, Tanaka N, Kunugi S
Laboratory for Biopolymer Physics, Department of Polymer Science and Engineering, Kyoto Institute of Technology, Matsugasaki, Sakyo, Kyoto 606-8585, Japan.
Biochim Biophys Acta. 1998 Dec 22;1443(3):364-8. doi: 10.1016/s0167-4781(98)00244-9.
By conducting topoisomerase I-mediating supercoiling assays, effects of elevated pressure on DNA supercoiling were investigated for the first time. It was found that pressure elevations induced a progressive increase in plasmid DNA linking numbers, winding the DNA duplex by a magnitude of 1.1-1.6x10(-3) angular degree/base/MPa. Implications for the findings were discussed in terms of disturbance of the tertiary structure of DNA by elevated pressure.
通过进行拓扑异构酶I介导的超螺旋分析，首次研究了高压对DNA超螺旋的影响。结果发现，压力升高导致质粒DNA连接数逐渐增加，使DNA双链以每兆帕1.1 - 1.6×10⁻³角度/碱基的幅度缠绕。从高压对DNA三级结构的干扰方面对这些发现的意义进行了讨论。
