Pajor L
Pécsi Orvostudományi Egyetem, Patológiai Intézet.
Orv Hetil. 1998 Dec 6;139(49):2939-46.
Nowadays, the detection of specific DNA sequences on interphase nuclei of cytological and paraffin slide preparations by in situ hybridization, the interphase cytogenetics became an established technology in the pathological diagnostics. A historical overview on the development of the technique is presented, the theoretical basis of the detection of numerical and structural chromosomal aberrations is demonstrated and the applications are exemplified on different types of malignant lymphomas, leukaemias as well as epithelial tumors. Combined use of the interphase cytogenetics, light microscopy and immunohistochemistry with the digital imaging techniques can provide us with morphological, immunophenotypic and genotypic informations of the same cellular object which might be a milestone in the pathomorphological diagnostics.
如今，通过原位杂交在细胞学和石蜡切片制备的间期核上检测特定DNA序列，使得间期细胞遗传学成为病理诊断中的一项成熟技术。本文介绍了该技术发展的历史概况，阐述了检测染色体数目和结构异常的理论基础，并举例说明了其在不同类型恶性淋巴瘤、白血病以及上皮性肿瘤中的应用。将间期细胞遗传学、光学显微镜检查和免疫组织化学与数字成像技术结合使用，可以为我们提供同一细胞对象的形态学、免疫表型和基因型信息，这可能是病理形态学诊断中的一个里程碑。
