Burioni R, Plaisant P, Bugli F, Delli Carri V, Candela M, Gabrielli A, Fadda G
Istituto di Microbiologia, Facoltà di Medicina, Università Cattolica del Sacro Cuore, Roma, Italy.
Res Virol. 1998 Sep-Oct;149(5):321-5. doi: 10.1016/s0923-2516(99)89013-x.
It is well known that immunoglobulins with no identifiable immunogenic origin, called natural antibodies, are present in the sera of healthy individuals and their role as a defence against important pathogens has been proposed. Unfortunately, the studies are hampered by the fact that these immunoglobulins seem to have low affinity and to be polyreactive, and are commonly available in polyclonal preparations. Lately, new technologies for the production of monoclonal antibodies became available, and in particular the cloning of genes coding for antibody fragments in combinatorial phage display vectors provided a handy tool for the selection of human monoclonal antibodies. In this work, we describe the successful development of a technology for the molecular cloning of combinatorial phage display libraries containing genes coding exclusively for antibody fragment of the IgM or IgD phenotype. These libraries can be useful for molecular cloning of monoclonal antibodies of the IgM and IgD phenotype and can help elucidate the role played by natural antibodies in defence against infectious agents.
众所周知，在健康个体的血清中存在着没有可识别免疫原来源的免疫球蛋白，即所谓的天然抗体，并且有人提出了它们作为抵御重要病原体的防御作用。不幸的是，这些研究受到以下事实的阻碍：这些免疫球蛋白似乎亲和力较低且具有多反应性，并且通常以多克隆制剂形式存在。最近，产生单克隆抗体的新技术问世，特别是在组合噬菌体展示载体中克隆编码抗体片段的基因，为筛选人单克隆抗体提供了便利工具。在这项工作中，我们描述了一种技术的成功开发，该技术用于分子克隆组合噬菌体展示文库，该文库仅包含编码IgM或IgD表型抗体片段的基因。这些文库可用于IgM和IgD表型单克隆抗体的分子克隆，并有助于阐明天然抗体在抵御感染因子中的作用。
