Loo D T, Bradford S, Helmrich A, Barnes D W
Bristol-Myers Squibb, Pharmaceutical Research Institute, Princeton, NJ, USA.
Cell Biol Toxicol. 1998 Dec;14(6):375-82. doi: 10.1023/a:1007518909429.
SFME cells are brain-derived neural precursor cells that are acutely dependent on epidermal growth factor (EGF) for survival, undergoing apoptosis within 24 h after EGF withdrawal. Because the expression of the protooncogene bcl-2 inhibits apoptosis induced by the withdrawal of interleukins or nerve growth factor in some growth factor-dependent haematopoietic or neuronal cell cultures, we examined the effect of Bcl-2 expression on cell death of SFME cells in the absence of EGF. SFME cells expressing human Bcl-2 showed prolonged survival when deprived of EGF compared to control cells not expressing Bcl-2. A significant fraction of Bcl-2-expressing cells remained viable for 4 days in the absence of EGF and resumed proliferation upon readdition of EGF to the cultures. These results suggest that apoptosis induced by EGF withdrawal in SFME cells may share common mechanisms with other growth factor-related apoptotic systems.
SFME细胞是源自大脑的神经前体细胞，对表皮生长因子（EGF）的存活具有急性依赖性，在EGF撤除后24小时内会发生凋亡。由于原癌基因bcl-2的表达在一些生长因子依赖性造血或神经元细胞培养物中可抑制因白细胞介素或神经生长因子撤除所诱导的凋亡，因此我们研究了在缺乏EGF的情况下Bcl-2表达对SFME细胞死亡的影响。与未表达Bcl-2的对照细胞相比，表达人Bcl-2的SFME细胞在缺乏EGF时存活时间延长。在缺乏EGF的情况下，相当一部分表达Bcl-2的细胞在4天内仍保持存活，并在向培养物中重新添加EGF后恢复增殖。这些结果表明，SFME细胞中EGF撤除所诱导的凋亡可能与其他生长因子相关的凋亡系统具有共同机制。
