Schmallenbach K H, Rahman I, Sasse H H, Dixon P M, Halliwell R E, McGorum B C, Crameri R, Miller H R
Department of Veterinary Clinical Studies, Royal (Dick) School of Veterinary Studies, University of Edinburgh, UK.
Vet Immunol Immunopathol. 1998 Dec 11;66(3-4):245-56. doi: 10.1016/s0165-2427(98)00202-5.
Inhalant exposure to Aspergillus fumigatus (Asp. f.) antigens induces marked inflammatory and immunological alterations in the lungs of horses affected with chronic obstructive pulmonary disease (COPD). In this study we investigated the role of specific allergen(s) present in Asp. f. on systemic and pulmonary IgE and IgG responses in control and COPD-affected horses, using an enzyme-linked immunosorbent assay (ELISA) and immunoblotting techniques. Compared with controls, horses affected with COPD had significantly higher levels of BALF IgE and IgG to somatic Asp. f. antigens as well as to the allergen 1/a (Asp. f. 1/a). Serum levels of IgE and IgG against these antigens did not differ between control and COPD-affected horses. Antigen specific IgE and IgG levels did not correlate between BALF and serum. Scanning of Asp. f. and IgE and IgG blots revealed bands that are recognised by both IgE- and IgG-specific antibodies. Additionally, all horses responded with BALF IgE- and IgG-specific for 93, 35, 31 and 23 kDa allergens, suggesting that these antigens are involved in the induction of airway IgE and IgG responses. These allergens may have the potential to be used as biomarkers for the diagnosis of Asp. f. related exacerbations of equine COPD.
吸入烟曲霉（Asp. f.）抗原会在患有慢性阻塞性肺疾病（COPD）的马匹肺部引发明显的炎症和免疫改变。在本研究中，我们使用酶联免疫吸附测定（ELISA）和免疫印迹技术，研究了Asp. f.中存在的特定过敏原对对照马匹和患COPD马匹全身及肺部IgE和IgG反应的作用。与对照相比，患COPD的马匹支气管肺泡灌洗液（BALF）中针对烟曲霉体细胞抗原以及过敏原1/a（Asp. f. 1/a）的IgE和IgG水平显著更高。对照马匹和患COPD马匹血清中针对这些抗原的IgE和IgG水平没有差异。抗原特异性IgE和IgG水平在BALF和血清之间不相关。对烟曲霉以及IgE和IgG印迹的扫描显示出能被IgE和IgG特异性抗体识别的条带。此外，所有马匹对93、35、31和23 kDa过敏原的BALF IgE和IgG均有反应，表明这些抗原参与了气道IgE和IgG反应的诱导。这些过敏原可能有潜力用作诊断与马COPD相关的烟曲霉加重情况的生物标志物。
