用从微小牛蜱卵中分离出的天冬氨酸蛋白酶前体对牛进行免疫接种。

Immunization of bovines with an aspartic proteinase precursor isolated from Boophilus microplus eggs.

作者信息

da Silva Vaz I, Logullo C, Sorgine M, Velloso F F, Rosa de Lima M F, Gonzales J C, Masuda H, Oliveira P L, Masuda A

机构信息

Centro de Biotecnologia do Estado do Rio Grande do Sul, and Faculdade de Veterinária, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil.

出版信息

Vet Immunol Immunopathol. 1998 Dec 11;66(3-4):331-41. doi: 10.1016/s0165-2427(98)00194-9.

DOI:10.1016/s0165-2427(98)00194-9

PMID:9880109

Abstract

The capacity of the Boophilus Yolk pro-Cathepsin (BYC) to induce a protective immune response in cattle against Boophilus microplus infestation was tested by vaccination experiments and by inoculation of monoclonal antibody (MAb) against BYC into fully engorged tick females. In immunization experiments the measurement of various biological parameters demonstrated a partial protection against B. microplus. A continuous decrease in the levels of specific antibodies was observed over 11 months when six bovines were maintained in field conditions. The inoculation of the MAb into tick females produced a dose-dependent decrease in oviposition and survival of the ectoparasite compared to the control.

摘要

通过疫苗接种实验以及将抗嗜牛蜱卵黄组织蛋白酶原（BYC）单克隆抗体（MAb）接种到饱血蜱雌虫体内，测试了嗜牛蜱卵黄组织蛋白酶原（BYC）诱导牛对微小牛蜱感染产生保护性免疫反应的能力。在免疫实验中，对各种生物学参数的测量表明对微小牛蜱有部分保护作用。当六头牛处于田间条件下时，在11个月内观察到特异性抗体水平持续下降。与对照组相比，将单克隆抗体接种到蜱雌虫体内使体外寄生虫的产卵量和存活率呈剂量依赖性下降。