用重组嗜牛蜱卵黄组织前组织蛋白酶对牛进行疫苗接种。
Vaccination of bovines with recombinant Boophilus Yolk pro-Cathepsin.
作者信息
Leal Alexandre T, Seixas Adriana, Pohl Paula C, Ferreira Carlos A S, Logullo Carlos, Oliveira Pedro L, Farias Sandra E, Termignoni Carlos, da Silva Vaz Itabajara, Masuda Aoi
机构信息
Centro de Biotecnologia, Universidade Federal do Rio Grande do Sul, Avenida Bento Gonçalves, 9500, Prédio 43421, Porto Alegre RS 91501-970, Brazil.
出版信息
Vet Immunol Immunopathol. 2006 Dec 15;114(3-4):341-5. doi: 10.1016/j.vetimm.2006.08.011. Epub 2006 Sep 25.
Boophilus Yolk pro-Cathepsin (BYC) is an aspartic proteinase found in Boophilus microplus eggs that is involved in the embryogenesis and has been tested as antigen to compose an anti-tick vaccine. The vaccine potential of a recombinant BYC expressed in Escherichia coli (rBYC) was investigated. rBYC was purified and used to immunize Hereford cattle. The sera of bovines immunized with rBYC recognized the native BYC with a titer ranging from 125 to 4000. Furthermore, immunized bovines challenged with 20,000 larvae presented an overall protection of 25.24%. The partial protection obtained against B. microplus infestation with the recombinant protein immunization was similar to the already described for native BYC immunization.
微小牛蜱卵原组织蛋白酶（BYC）是一种在微小牛蜱卵中发现的天冬氨酸蛋白酶，它参与胚胎发生，并已作为抗原进行测试以制备抗蜱疫苗。研究了在大肠杆菌中表达的重组BYC（rBYC）的疫苗潜力。rBYC被纯化并用于免疫赫里福德牛。用rBYC免疫的牛血清识别天然BYC，效价范围为125至4000。此外，用20000只幼虫攻击的免疫牛总体保护率为25.24%。重组蛋白免疫对微小牛蜱感染获得的部分保护与已描述的天然BYC免疫相似。
Zotero 插件