多杀巴斯德菌孔蛋白对小鼠脾细胞细胞因子表达和释放的作用

Role of Pasteurella multocida porin on cytokine expression and release by murine splenocytes.

作者信息

Iovane G, Pagnini P, Galdiero M, Cipollaro de l'Ero G, Vitiello M, D'Isanto M, Marcatili A

机构信息

Dipartimento di Patologia, Profilassi ed Ispezione degli Alimemti, Sezione Malattie Infettive, Facoltà di Veterinaria, Università degli Studi di Napoli Federico II, Naples, Italy.

出版信息

Vet Immunol Immunopathol. 1998 Dec 11;66(3-4):391-404. doi: 10.1016/s0165-2427(98)00183-4.

DOI:10.1016/s0165-2427(98)00183-4

PMID:9880114

Abstract

The aim of this study was to verify whether Pasteurella multocida porin can affect the expression and release of IL-1alpha, IL-6, TNF-alpha, IL-4, IFN-gamma, IL-10 and IL-12 by murine splenocytes in vitro. P. multocida porin and lipopolysaccharide (LPS) were able to induce the release of IL-1alpha, IL-6, TNF-alpha, IFN-gamma and IL-12 in a dose-dependent fashion. The greatest release of these cytokines was obtained using P. multocida porin at a concentration of 5 microg ml(-1) and LPS at a concentration of 1 microg ml(-1). The time-courses of release showed that P. multocida LPS was able to stimulate the production of IL-1alpha, IL-6, TNF-alpha, IFN-gamma and IL-12 earlier than porin and at a greater rate. No effect was observed on IL-4 and IL-10 release under the same experimental conditions. P. multocida porin and LPS were also able to up-regulate the mRNA expression of IL-1alpha, IL-6, TNF-alpha, IFN-gamma and IL-12 p40. Our findings suggest that P. multocida porin is able to modulate inflammatory and immunological responses by affecting the release of several cytokines and the expression of their genes.

摘要

本研究的目的是验证多杀巴斯德菌孔蛋白是否能在体外影响小鼠脾细胞中白细胞介素-1α（IL-1α）、白细胞介素-6（IL-6）、肿瘤坏死因子-α（TNF-α）、白细胞介素-4（IL-4）、干扰素-γ（IFN-γ）、白细胞介素-10（IL-10）和白细胞介素-12（IL-12）的表达与释放。多杀巴斯德菌孔蛋白和脂多糖（LPS）能够以剂量依赖的方式诱导IL-1α、IL-6、TNF-α、IFN-γ和IL-12的释放。使用浓度为5微克/毫升的多杀巴斯德菌孔蛋白和浓度为1微克/毫升的LPS时，这些细胞因子的释放量最大。释放的时间进程表明，多杀巴斯德菌LPS比孔蛋白更早且以更高的速率刺激IL-1α、IL-6、TNF-α、IFN-γ和IL-12的产生。在相同实验条件下，未观察到对IL-4和IL-10释放的影响。多杀巴斯德菌孔蛋白和LPS还能够上调IL-1α、IL-6、TNF-α、IFN-γ和IL-12 p40的mRNA表达。我们的研究结果表明，多杀巴斯德菌孔蛋白能够通过影响多种细胞因子的释放及其基因表达来调节炎症和免疫反应。