生长素处理的豌豆茎质外体中纤维寡糖的出现。
Occurrence of cello-oligosaccharides in the apoplast of auxin-treated pea stems.
作者信息
Tominaga R, Samejima M, Sakai F, Hayashi T
机构信息
Wood Research Institute, Kyoto University, Gokasho Uji, Kyoto 611, Japan (R.T., F.S., T.H.).
出版信息
Plant Physiol. 1999 Jan;119(1):249-54. doi: 10.1104/pp.119.1.249.
Treatment of pea (Pisum sativum L.) hypocotyl segments with indole-3-butyric acid, which promotes segment elongation, increased the solubilization of both xyloglucan and cello-oligosaccharides in the apoplast of auxin-treated pea stems. The cello-oligosaccharides were isolated from the apoplastic solution with a charcoal/Celite column and were identified as cellobiose, cellotriose, and cellotetraose after subsequent thin-layer chromatography and paper electrophoresis. Cello-oligosaccharides in the apoplastic fraction were monitored using cellobiose dehydrogenase. Both xyloglucan and cello-oligosaccharides appeared to be formed concurrently within 30 min after treatment with the auxin, and the cello-oligosaccharides increased with stem elongation even after 2 h. The total activity of cellulase did not increase for up to 4 h.
用吲哚 - 3 - 丁酸处理豌豆（Pisum sativum L.）下胚轴切段，该处理可促进切段伸长，同时增加了生长素处理的豌豆茎质外体中木葡聚糖和纤维寡糖的溶解。用活性炭/硅藻土柱从质外体溶液中分离出纤维寡糖，随后通过薄层色谱和纸电泳鉴定为纤维二糖、纤维三糖和纤维四糖。使用纤维二糖脱氢酶监测质外体部分中的纤维寡糖。木葡聚糖和纤维寡糖似乎在生长素处理后30分钟内同时形成，即使在2小时后，纤维寡糖也随着茎的伸长而增加。纤维素酶的总活性在长达4小时内没有增加。
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