Bussmann V, Lantier I, Pitel F, Patri S, Nau F, Gros P, Elsen J M, Lantier F
Pathologie Infectieuse et Immunologie, INRA de Tours-Nouzilly, 37380 Nouzilly, France.
Mamm Genome. 1998 Dec;9(12):1027-31. doi: 10.1007/s003359900919.
Mouse resistance to several intracellular pathogens including Mycobacteria, Leishmania, and Salmonella is under the control of the Chromosome (Chr) 1 Natural Resistance Associated Macrophage Protein I gene (Nramp1). This gene could have an economic and health importance for domestic animals and humans as well. Therefore, equivalents of the NRAMP1 gene have been cloned by several research groups in various animal species. To study in sheep the influence of the NRAMP1 gene on the susceptibility to intracellular pathogens induced diseases, we have cloned the sheep NRAMP1 cDNA by screening a splenic cDNA library. The genomic organization of the sheep NRAMP1 gene was then determined by sequencing the exon/intron boundaries. The transcription start points (tsp) from the NRAMP1 mRNA have been located with primer extension experiments. RT-PCR reactions have been used to determine the profile of mRNA expression of this gene.
小鼠对包括分枝杆菌、利什曼原虫和沙门氏菌在内的几种细胞内病原体的抗性受1号染色体天然抗性相关巨噬细胞蛋白I基因（Nramp1）的控制。该基因对家畜和人类也可能具有经济和健康方面的重要性。因此，多个研究小组已在各种动物物种中克隆了NRAMP1基因的等效物。为了研究绵羊中NRAMP1基因对细胞内病原体诱发疾病易感性的影响，我们通过筛选脾脏cDNA文库克隆了绵羊NRAMP1 cDNA。然后通过对外显子/内含子边界进行测序确定了绵羊NRAMP1基因的基因组结构。通过引物延伸实验确定了NRAMP1 mRNA的转录起始点（tsp）。已使用RT-PCR反应来确定该基因的mRNA表达谱。
